A Lateral Flow Assay for Quantitative Detection of Amplified HIV-1 RNA
Figure 3
Performance of the lateral flow assay after storage.
To assess the effects of storage on LFA performance, lateral flow strips were fabricated on the same day, placed in foil pouches with desiccant, and stored at room temperature or 37°C. The lateral flow assay was performed and analyzed on the day of strip creation and 1, 3, 7, 14, 21, and 28 days after strip creation. The signal-to-background ratio (SBR) for each log10 copy number is shown for strips performed on different days. The negative control SBR is shown for comparison. The regression line and equation were calculated for the average SBRs over the linear range of the assay, from 10.5 to 13 log10 RNA copies. (A) Dose response curves at each time point and (B) average dose response curve for strips stored at room temperature. (C) Dose response curves at each time point and (D) average dose response curve for strips stored at 37°C.