Roles of Dopamine 2 Receptor Isoforms and G Proteins in Ethanol Regulated Prolactin Synthesis and Lactotropic Cell Proliferation
Figure 5
Effects of dopamine and pertussis toxin on ethanol modulated cellular levels of PRL and cell proliferation in D2S (A, D, G), D2L (B, E, H) and V cells (C, F, I).
Cells were treated with the dopamine (DA) or pertussis toxin (PTX) as described in the legend of Fig. 4. Cellular levels of PRL were determined by Western blots (A–C) or by ELISA (D–F). Cell proliferation studies were conducted by measuring the [3H]thymidine incorporation into cells (G–I). N = 4–9. *, **,***, P<0.05, P<0.01, P<0.001, respectively as compared to the vehicle-treated group (C). a, P<0.05, significantly different from the EtOH only treatment.