Urochordate Ascidians Possess a Single Isoform of Aurora Kinase That Localizes to the Midbody via TPX2 in Eggs and Cleavage Stage Embryos
Figure 5
Ci-Aurora::Venus localization in sea urchins.
Ci-Aurora::Venus and HH2B::Rfp1 mRNA were injected into unfertilized sea urchin eggs (Paracentrotus lividus) and the eggs subsequently fertilized. During cleavage cycles Ci-Aurora::Venus and HH2B::Rfp1 proteins are produced and become fluorescent. Confocal images from a time series of one mitotic cleavage cycle are depicted (at 0, 8, 18, 24 min.). Ci-Aurora::Venus localizes to the nucleus (green arrow), chromosomes (yellow arrow), the spindle pole (blue arrow) and finally to the central spindle and midbody (red arrow). Corresponding brightfield and merged images are shown (red is HH2B::Rfp1 and green Ci-Aurora::Venus). Scale bar is 10 µm. n = 16, 3 animals.