Hedgehog Pathway Signaling Regulates Human Colon Carcinoma HT-29 Epithelial Cell Line Apoptosis and Cytokine Secretion
Figure 6
Relationship between apoptosis and the activity of Hedgehog pathway in HT-29 cells.
Flow cytometric demonstration of HT-29 apoptosis after 24 hours of exposure to different stimuli, as assessed by annexin-V/7-AAD. Left lower quadrant indicates double negative cells while right lower quadrant indicates annexin-V-positive cells (A). Treatment with Shh, purmorphamine or butyrate significantly abrogates the anti-CD95 induced apoptosis (*P<0.02), which is partially restored by the addition of cyclopamine (B). Data are expressed as the mean ± SEM of 3 independent experiments.