Microbial Distribution and Abundance in the Digestive System of Five Shipworm Species (Bivalvia: Teredinidae)
Figure 5
Bacterial morphotypes in the intestine of Bankia setacea visualized by single probe hybridization.
Confocal micrographs depicting hybridization of either a bacteria-domain specific 16S rRNA directed oligonucleotide probe (Bact338-cy5) or a negative control probe (Non338-cy5) to sectioned tissues of Bankia setacea. Both probes are labeled with the fluorochrome cy5 shown in red. (A) Tissue section hybridized with Bact338-cy5, showing a fecal pellet (F) with a small portion of the intestinal wall (I) visible in the lower left corner. Note the presence of multiple cell morphologies including long spirals (large arrow) and short rods or cocci (small arrow). (B) Enlarged view of the boxed region in A. (C) Tissue section adjacent to and comparable in size to that shown in (A) hybridized with Non338-cy5. Scale bars are 10 µm in (A) and (C) and 5 µm in (B).