Dynamin 1 Regulates Amyloid Generation through Modulation of BACE-1
Figure 1
Dynamin 1 (dyn1) knockdown of in N2a 695 cells reduces Aβ and βCTF levels.
A) N2a 695 cells were treated with dyn1 or control siRNA for analysis of APP metabolism. Levels of media and intracellular Aβ, sAPPβ, βCTF and holoAPP were determined by immunoprecipitation with 4G8 or MAB348 followed by immunoblotting with 6E10 or sAPPβ antibody. Recombinant sAPPβ peptide was used as a positive control. Levels of β-actin were also determined. B) Protein levels were normalized to β-actin and expressed as percentage of control. Data were collected in duplicate or triplicate from three independent experiments. Significant reductions (**p<0.001) in Aβ, βCTF and sAPPβ (*p<0.05) were observed upon dyn1 knockdown, as compared to control. Levels of Aβ40 and Aβ42 in the media are determined by sandwich ELISA analysis (**p<0.001).