Annexin A6 Modulates Chick Cranial Neural Crest Cell Emigration
Figure 2
A 5 base pair mismatch Annexin A6 control MO does not affect chick midbrain neural crest cell emigration in vivo.
(A) Immunoblots for Annexin A6 and β-actin showing reduced levels of Annexin A6 protein upon Annexin A6 MO treatment, with no change observed upon control MO treatment. (B,D) Whole-mount in situ hybridization followed by indicated transverse sections for Sox10 (C) and Snail2 (E), respectively, after 8 hour incubation following treatment with control MO. (F) Representative transverse section taken from an embryo treated with control MO for 8 hours followed by immunohistochemistry for HNK-1 (green). No change is noted in whole-mount images or sections with respect to neural crest cell emigration or migration. In all experiments, the right side of the embryo is electroporated, as indicated by the lissamine (red) fluorescence of the MO in the transverse sections (C,E) and/or in the inset images of each whole-mount (B,D). Scale bar in (B) is 50 µm and applicable to all whole-mount and section images. MO, red; DAPI, blue.