Annexin A6 Modulates Chick Cranial Neural Crest Cell Emigration
Figure 1
Annexin A6 transcript distribution in the developing chick embryo.
(A,C,E,G,I) Whole-mount in situ hybridization for Annexin A6 followed by indicated transverse sections (B,D,F,H,J) shown for specific developmental stages (A,B: 4 ss; C,D: 5 ss; E,F: 6 ss; G,H: 7 ss; I–J: 8 ss). (K) Representative midbrain transverse section taken from an 8 ss embryo that has undergone whole-mount in situ hybridization for Annexin A6 followed by sectioning and immunohistochemistry for the migratory neural crest cell marker HNK-1 (red). Arrowheads in (F,H,J,K) indicate Annexin A6 mRNA in migratory neural crest cells. Scale bars in (A) and (B) are 200 and 50 µm, respectively, and are applicable to respective image types.