Arabidopsis bHLH100 and bHLH101 Control Iron Homeostasis via a FIT-Independent Pathway
Figure 2
Expression of iron-responsive genes in fit-2/35S::FIT:GR plants.
8-day-old plants were grown on 1/2 MS without iron (black) or supplemented with 100 µM Fe (white). Roots were then transferred to liquid media with the same iron content but supplemented as follows: CHX DEX: 100 µM cycloheximide for one hour then 30 µM dexamethasone was added and incubated for 3 hours; CHX: 100 µM cycloheximide for one hour followed by a mock treatment for 3 hours, DEX: mock treated for one hour followed by 30 µM dexamethasone for 3 hours; or mock treatments only. Quantitative RT-PCR results are presented for IRT1 (A) FRO2 (B) bHLH038 (C) and bHLH100 (D). Error bars indicate standard error (n = 3–4). Relative transcript levels are shown on the graph.