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Combination of Multiple Resistance Traits from Wild Relative Species in Chrysanthemum via Trigeneric Hybridization

Figure 4

GISH analysis of the trigeneric hybrid line T3.

(A) All 54 chromosomes showing blue fluorescence after staining with DAPI. (B) Among the 54 chromosomes, nine fluoresced green after staining with the probe for Crossostephium chinense genomic DNA, and nine fluoresced red after staining with the probe for Artemisia vulgaris genomic DNA; thus the other 36 chromosomes were obtained from C. grandiflorum ‘Zhongshanjingui’ and/or C. crassum. Bars: 5 µm.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0044337.g004