Characterization of a Novel Phenol Hydroxylase in Indoles Biotranformation from a Strain Arthrobacter sp. W1
Figure 5
Summary of transformation pathways of indole by various oxygenases.
DO. dioxygenase; SO. styrene oxygenase; MO. monooxygenase. R represents for substitute group i.e. methyl-, chloro-, methyoxy-, etc. The pathways catalyzed by strain PH_IND could be proposed by C-3 oxidation and C-7 oxidation pathways. The indole is firstly hydroxylated at the C-3 positions to form indoxyl (3-hydroxyindole) by strain PH_IND, which undergoes further oxidation to form isatin as well as the indigoids precursors. Finally, two molecules of indoxyl polymerize to form indigo, and indoxyl can form indirubin with isatin. And the new compound is formed by 7-hydroxyindole and isatin.