Characterization of a Novel Phenol Hydroxylase in Indoles Biotranformation from a Strain Arthrobacter sp. W1
Figure 1
SDS-PAGE analysis of protein samples of PH_IND from E. coli BL21 (DE3).
Line 1. Whole cells of pET-28a(+); Line 2. Cell extracts of pET-28a(+); Line 3. Precipitation of pET-28a(+); Line 4. Whole cells of strain PH_IND; Line 5. Cell extracts of strain PH_IND; Line 6. Precipitation of strain PH_IND; M. Protein markers. Arrows show the positions of the six ORFs. ORF1. 10.4 kDa; ORF2. 37.6 kDa; ORF3. 10.5 kDa; ORF4. 59.2 kDa; ORF5. 13.5 kDa; ORF6. 38.6 kDa. SDS-PAGE was performed with 5% and 15% acrylamide concentrations for the concentrating and separating gels, respectively.