Quantitative Modeling Assesses the Contribution of Bond Strengthening, Rebinding and Force Sharing to the Avidity of Biomolecule Interactions
Figure 3
Lifetime of binding events recorded on surfaces coated with monovalent ICAM-1.
The figure shows the survival curves of binding events recorded between surfaces coated with low densities of monovalent ICAM-1 and microspheres coated with anti-ICAM-1 antibodies. Red, squares: wall shear rate 9.3 s−1, microsphere velocity 11.25 µm/s, 47 binding events recorded. Green, triangles: wall shear rate 18.5 s−1, microsphere velocity 22.5 µm/s, 1,725 binding events recorded. Blue, circles: wall shear rate 29.4 s−1, microsphere velocity 35.7 µm/s, 936 binding events recorded. Fig. 3A: the raw values were used. Fig. 3B: values were corrected to account for nonspecific events as explained. The curves represented the best fits of experimental curves with Eq. 2. Squares: Force on bond is 8.37 pN, k(F,0) = 0.441 s−1, a(F) = 1.099 s−1, red line: calculated fit, MSD = 3.7 10−3. Crosses: Force on bond is 16.75 pN, k(F,0) = 1.735 s−1, green line: calculated fit, MSD = 0.99 10−3. Circles: Force on bond is 26.61 pN, k(F,0) = 4.603 s−1, a(F) = 6.149 s−1, MSD = 12.4 10−3. Vertical bar length is twice standard error.