Vacuolar Protein Sorting Receptor in Giardia lamblia
Figure 5
Subcellular distribution of GlVps.
(A) Direct IFA and confocal microscopy show that GlVps-HA (green) partially colocalizes with the ER-resident chaperone BiP (red) in the ER (Merge in yellow). Inset magnifies a region of the cell and shows the green and red fluorescence in the ER (a). Differential interference contrast microscopy (b) is shown as insert. Scatter plot of the two labels confirms the colocalization (right panel). (B) Direct IFA and confocal microscopy using the 2F5 mAb that detect the μ2 subunit of AP2 in the PVs (red) and anti-HA mAb to detect GlVps-HA (green), show the presence of GlVps-HA in some PVs. Inset magnifies a region of the cell where the green and red fluorescence partially overlap (a). Differential interference contrast microscopy (b) is shown as insert. Bar, 10 μm. Scatter plot (panel on the right) correspond to the colocalization analysis. Pearson's coefficient (PC). Manders' Overlap coefficient (M).