Vacuolar Protein Sorting Receptor in Giardia lamblia
Figure 1
AcPh localization and activity.
(A) Schematic representation of the acph gene containing the GGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGCGTA. CCGGT and CATCATCATCATCATCAT, coding to the V5 epitope and six histidine residues, respectively. A 3D reconstruction of the gene product tagged with V5-H6 using the hidden Markov models (HMMs) [84] and MODELLER [85] is also represented. (B) IFA and confocal microscopy show AcPh-V5 predominantly in the ER but also in the nuclear envelope and PVs (arrowheads). DIC: Differential interference contrast microscopy. (C) Acid phosphatase activity on the PVs and bare zone is observed by using the specific substrate ELF97 at pH 5.5. Alkaline phosphatase activity was not detected in trophozoites at pH ≥7.0. Nuclear DNA was labeled with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Bar, 10 μm.