Endogenous Fluorescence Signatures in Living Pluripotent Stem Cells Change with Loss of Potency
Figure 4
Global endogenous fluorescence intensity and lifetime changes observed with differentiation of human EBs.
A. Background subtracted intensity of MPLSM single optical sections of the same live hEB at 5, 7, 10 and 16 days post-EB formation, using 780 nm excitation. B. Color mapped fluorescence lifetime (τm) of the same hEB as in upper row, over time. Color bar indicates lifetime values for τm. Scale bar = 50 µm. C. Quantitation shows changes in mean fluorescence intensity at the different time points (bars: for days 5, 7, 10, 16 n = 16, 17, 17, 16 EBs, respectively) while colored lines indicate the changes in intensity of individual EBs tracked over time. D. Quantitation of τm, with bar graph showing the mean τm at the given time points (for days 5, 7, 10, 16 n = 5, 6, 6, 5 EBs, respectively), while colored lines show the changes in lifetime of individual hEBs tracked over time. Horizontal bars indicate differences at P<0.05 (Holm-Sidak method for pairwise comparisons following repeated measures ANOVA). Quantitation of additional lifetime parameters and detailed statistics in Figure S6.