Endogenous Fluorescence Signatures in Living Pluripotent Stem Cells Change with Loss of Potency
Figure 2
Lactate production and metabolic substrate requirements change with differentiation.
A. Lactate production as detected in the medium on days 3 through 8 of mEB development. Horizontal lines indicate statistical difference (P<0.05) (Holm-Sidak method pairwise multiple comparison following repeated measures ANOVA). B. Percentage of mEBs that exhibited beating areas when cultured in either standard medium (Glu & Pyr), medium with glucose only (Glu) or medium with pyruvate only (Pyr) as assessed on days 8, 9, 10 and 12 after EB formation. Horizontal lines indicate statistical difference (P<0.05) (Tukey test following ANOVA). C. Quantitation of cTnT antibody labeling using fluorescence intensity measures from EBs grown with glucose and pyruvate, glucose only or pyruvate only. Horizontal lines indicate statistical difference (P<0.05) (Tukey pairwise comparison test following ANOVA). D. Examples of cTnT antibody labeling in EBs cultured in the 3 media. Scale bar = 500 µm.