Enhanced Vitellogenesis in a Whitefly via Feeding on a Begomovirus-Infected Plant
Figure 1
Elution profile of crude vitellin from UNOTMQ1 ion exchange column.
Vitellin was eluted with a gradient of 0.0–1.0 M NaCl in Tris–HCl buffer pH 7.8 (A); fractions indicated with arrow were pooled as purified Vt, verified using SDS–PAGE (B, left) and Western blot (B, right) with monoclonal antibodies against B. tabaci Vt. E: purified Vt; PM: prestained molecular weight standards.