Golgi Cell-Mediated Activation of Postsynaptic GABAB Receptors Induces Disinhibition of the Golgi Cell-Granule Cell Synapse in Rat Cerebellum
Figure 7
Activation of postsynaptic GABAB receptors is sufficient to inhibit GABAA receptor-mediated responses in granule cells. A,
To examine the effects of GABAB receptor activation in a granule cell in the absence of Golgi cell stimulation, synaptic transmission was blocked by superfusing slices with TTX (1 µM) and GABAA receptor-mediated responses were elicited by puff applications of muscimol. Averaged responses to muscimol are shown at 1 min (c1) and 5 min (c2) after establishing the whole cell configuration and at 10 min (p1), 20 min (p2), and 40- min (p3) after bath-application of baclofen. B, Histograms show averaged data for the effects of baclofen on muscimol-induced responses (n = 4). C, Time course of responses from a single cell, and D, pooled data for the effect of baclofen on muscimol-induced GABAA receptor-mediated currents.