Global Genome Analysis of the Downstream Binding Targets of Testis Determining Factor SRY and SOX9
Figure 2
ChIP-PCR confirmation of Sox9 as a downstream direct target of SRY.
Sox9 hybridization signals were not detected as the binding of SRY to Sox9 promoter is shown to occur at the TESCO region located at −7K upstream of the transcription start site. Non immune IgG was used as a negative control for each assay. ChIP DNA from IgG represented negative control throughout the experiment. PCR was conducted on 200 ng DNA amplified by whole genome amplification kit (Sigma). Data represent ChIP-PCR assay from three different experiments and biological replicates.