PKH26 Staining Defines Distinct Subsets of Normal Human Colon Epithelial Cells at Different Maturation Stages
Figure 4
Comparison by qRT-PCR of Stemness and Differentiation Marker Expression in Cultured Colon Cells and Microdissected Colon Crypt Populations.
mRNA levels of Lgr5, Msi-1 and CK20 genes were evaluated by qRT-PCR on FACS-sorted PKHhigh, PKHlow and PKHneg cells at the 3rd week of culture (Panel A) and on Differentiated (Diff), TA and CBC cells (Panel C) isolated by laser microdissection from colon crypts (as shown by the picture reported in Panel B). Data are shown as mean values ± SD calculated as described in detail in Materials and Methods, normalized to the housekeeping gene β2-microglobulin and relative to the reference sample (PKHlow for sorted cultured cells, and TA for cells isolated by microdissection). * p<0.05.