AAV-Mediated Knock-Down of HRC Exacerbates Transverse Aorta Constriction-Induced Heart Failure
Figure 2
Electrical- and caffeine-induced Ca2+ transients in NRVCs.
After 48 h of transfection with siNC or siHRC oligonucleotide, the NRVCs were treated with Fura 2-AM, and Ca2+ transients were measured at 1-Hz electrical stimulation or 40 mM caffeine application in Tyrode solution to measure sarcoplasmic reticulum (SR) Ca2+ load using IonOptix. A: Typical records of electrical- and caffeine-induced Ca2+ transients in siNC and siHRC oligonucleotide treated NRVCs. B: Significantly changed parameters of Ca2+ transients after HRC-KD. C: Left, the SR Ca2+ load was not significantly different between siNC and siHRC oligo-transfected NRVCs Right, the fractional Ca2+ release was significantly increased in HRC-KD NRVCs (siHRC). 16 sets of siNC and 21 sets of siHRC NRVCs were used for statistical analyses. Values are means ± S.E. *P<0.05.