The Effects of Glycogen Synthase Kinase-3beta in Serotonin Neurons
Figure 5
Regulation of serotonin release by 5-HT1B and 5-HT1A receptor agonists in the cerebral cortical slices.
(A) Potassium chloride (KCl, 50 mM)-evoked [3H]-serotonin release was measured in freshly isolated cerebral cortical slices of WT and snGSK3β-KO mice. Mean ± SEM, n = 22 individual treatment. (B) Serotonin release was stimulated by potassium chloride (KCl, 50 mM) twice. Prior to the second KCl stimulation, slices were treated with anpirtoline (Anp, 5 µM) or 8-OH-DPAT (DPAT, 1 µM) for 5 min. Data is expressed as percent of baseline [3H]-serotonin release (fractions 1–3). Mean ± SEM, n = 6–13 in each treatment. *p<0.05, **p<0.01 in LSD post hoc of two way ANOVA when treatment was compared to baseline in the same genotypes. The response to anpirtoline in WT and snGSK3β-KO mice was significantly different (p<0.05).