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Ruler Arrays Reveal Haploid Genomic Structural Variation

Figure 4

The ruler array analysis recognizes structural variants by fitting line segments to the microarray data and detecting differences in those segments between channels.

(a) The observed Ruler Array intensities (red is S288c and green is 1278b) at TRP1 reveal the presence of the synthetic 1182 bp insertion (bacterial HISG) in this 1278b trp- strain. The adjacent segment, which contains GAL3 and no differences between 1278b and S288c, shows no differences between strains and the two channels’ intensities track very closely to each other. (b) The dashed black lines show the fitted segments at TRP1, emphasizing the insertion’s position by the difference in the segments fitted to the two channels. The sequences of the tiling array probes are from the S288c genome.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0043210.g004