A Genome-Wide Over-Expression Screen Identifies Genes Involved in Phagocytosis in the Human Protozoan Parasite, Entamoeba histolytica
Figure 7
Phagocytosis is reduced in E. histolytica cells over-expressing H644 or EhLimA.
Wild-type (WT) or transgenic cells were incubated with erythrocytes (hRBC:amoeba ratio; 100∶1) for 10 minutes, lysed, and spectrophotometrically analyzed for internalized heme at 405 nm. The data represent the mean ± S.D. of 4 experiments (**P<0.01; *P<0.05). Amoebae over-expressing H644 or EhLimA exhibit reduced phagocytosis of hRBCs.