Dihydroartemisinin Exerts Its Anticancer Activity through Depleting Cellular Iron via Transferrin Receptor-1
Figure 6
The effects of DHA were partially dependent on TfR1 internalization.
(A) HepG2 cells were preincubated with 25 µg/ml nystatin or not for 30 min and treated with 25 µM DHA for another 4 hours. Cells were lysed to western blot assay. (B) HepG2 cells were preincubated with 25 µg/ml nystatin or not for 30 min and treated with 25 µM DHA for another 24 hours. Total RNA was harvested and quantitative RT-PCR was performed. *, P<0.05. Data are represented as mean ± SEM of more than three different experiments. (C) HepG2 cells were treated as (B) and lysed to western blot assay. (D) After transfected with 30 nM siRNA for 24 hours, HepG2 cells were treated with DHA for another 24 hours. Cell viability was determined via MTT assay. *, P<0.05; **, P<0.01. Data are represented as mean ±SD of three different experiments.