A Role of Tomato UV-Damaged DNA Binding Protein 1 (DDB1) in Organ Size Control via an Epigenetic Manner
Figure 1
Expression analysis of transcript variants of DDB1.
(A) Schematic diagram of DDB1 gene splicing pattern. Introns (I) and exons (E) are marked by lowercase and uppercase letters, respectively. Numbers above the letter and arrows indicate the position of the nucleotide in the genome sequence and the site of alternative splicing, respectively. The extra 15 bp nucleotides of DDB1+15 transcript are shown in grey. (B) The percentage of two DDB1 transcripts in different tissues. SD: seedlings; R: roots; SM: stems; L: leaves; F: flowers; IG1: immature green fruits at 7-DPA; IG2: immature green fruits at 14-DPA; MG: mature green fruits at 35-DPA. The light gray and black bars indicate the expression percentage of the DDB1+15 and DDB1F transcripts, respectively. Data presented are the means of three technical replicates. Similar results were obtained in at least two independent experiments. (C) Identification of the percentage of two DDB1 transcripts in seedlings under hormones or stress treatment. Total RNAs were extracted from 10-day-old seedlings with application of abscisic acid (ABA), auxin (indole acetic acid; IAA), cytokinin (6-benzylaminopurine, BA), gibberellins (GA) and mannitol. 10-day-old seedlings without any hormone and stress treatment were used as control. The light gray and black bars indicate the expression percentage of the DDB1+15 and DDB1F transcripts, respectively.