Tungstate Reduces the Expression of Gluconeogenic Enzymes in STZ Rats
Figure 4
Inhibitory effects of tungstate on gluconeogenesis in vitro.
Total mRNA was extracted from primary cultured rat hepatocytes treated with Dexamethasone 100 nM (Dex), sodium tungstate 1 mM (W) or both (Dex+W) for 18 h as indicated, and Real time-PCR was performed. Tungstate treatment increased the expression of c-jun and c-fos alone and in the presence of dexamethasone, whereas PGC-1α mRNA levels were decreased under these two conditions (A). Conversely, both PEPCK and G6Pase were downregulated after the treatment with tungstate alone (W) or in the presence of dexamethasone (Dex+W) (B). C Glucose output was measured in primary cultured hepatocytes incubated with the gluconeogenic substrates lactate (L) and pyruvate (P), as indicated. Data are representative of three experiments performed in triplicate. Error bars represent S.E.M. *, P<0.05; **, P<0.01; ***, P<0.001.