Tungstate Reduces the Expression of Gluconeogenic Enzymes in STZ Rats
Figure 2
Tungstate-induced changes in protein phosphorylation.
Phosphorylation of ERK, p90rsk, PKB and GSK3αβ was analyzed by Western blot using phospho-specific antibodies. Total ERK, total p90rsk, total PKB, total GSK3αβ and GAPDH were used as loading controls. Protein expression and phosphorylation were quantified by densitometry of the corresponding Western blot signal. A The images are representative of 3 controls (Ctrol), 3 controls treated with tungstate (Ctrol+W), 4 diabetic animals (Diab), and 4 diabetic animals treated with tungstate (Diab+W) from two independent experiments. In each experiment n = 6 for Ctrol, n = 7 for Ctrol+W, n = 9 for Diab and n = 14 for Diab+W. 20 µg of protein were loaded per well. Analysis of PEPCK expression in the same experimental groups serves as a control of tungstate action in those samples. B Bar graphs represent relative density (phospholylated versus total; PEPCK versus GAPDH) from Western blot signal (n = 3–8 in each group). Error bars represent S.E.M. *, P<0.05; **, P<0.01; ***, P<0.001.