DMH1, a Novel BMP Small Molecule Inhibitor, Increases Cardiomyocyte Progenitors and Promotes Cardiac Differentiation in Mouse Embryonic Stem Cells
Figure 4
DMH1 treatment does not specifically induce other mesoderm-associated lineage markers.
CGR8 induction and gene expression analysis were performed as previously described. The data show DM or DMH1 treatment does not specifically induce hematopoietic markers Gata1 and Flk-1/Kdr, or smooth muscle marker Myh11 when compared with DMSO control. Both compounds show a modest induction of vascular endothelial marker VE-CAM but the differences are not statistically significant. Results are presented as the average of three independent experiments. Expression levels shown are normalized to Day 0 expression levels. Error bars denote the S.E.M. DMSO is the vehicle control. DM is dorsomorphin treatment. DMH1 is dorsomorphin homologue 1.