Matrix metalloproteinase-2 Promotes αvβ3 Integrin-Mediated Adhesion and Migration of Human Melanoma Cells by Cleaving Fibronectin
Figure 4
MMP-2 activity affects the spreading of human A375 melanoma cells.
(A) The morphology of A375 cell spreading at different time points were designated as round, partially spread and completely spread. (B) Cells were seeded onto coverslips coated with fibronectin, treated with MMP-2I at the concentrations of 0.5 nM at different time points after seeding, and assessed under the microscope. The cells without MMP-2I were defined as control. The differences of cell morphology at 3 h, 6 h and 9 h after seeding were observed and counted (n≥400).