Micro-RNA-195 and -451 Regulate the LKB1/AMPK Signaling Axis by Targeting MO25
Figure 2
In situ hybridization of miR-195 and miR-451 expression in R403Q HCM hearts.
In situ hybridization of miR-195 and miR-451 in the left ventricle of male R403Q HCM hearts. 5′ digoxin-conjugated LNA-modified DNA probes complementary to mature miR-195 or -451 were used to detect native miR-195 and -451. Panel A (100X magnification): Scrambled sequence, missense 5′ digoxin-conjugated LNA-modified DNA probe; Panel B (100X magnification): miR-195, 5′ digoxin-conjugated LNA-modified miR-195 sequence probe; Panel C (100X magnification): miR-451, 5′ digoxin-conjugated LNA-modified miR-451 sequence probe. Insets: 200 X magnification. No significant signal was detected in hearts probed with the scrambled sequence. Sections treated with probes specific for miR-195 or miR-451 demonstrated a detectable signal.