Temporal Separation of Aggregation and Ubiquitination during Early Inclusion Formation in Transgenic Mice Carrying the Huntington’s Disease Mutation
Figure 4
Aggregate appearance in different brain regions over time.
Htt aggregates visualised by staining with the MW8 antibody (shown here at 14, 19, 22, 24, 26, 29, 41 and 63 days) in indisium griseum (IG), CA1, CA3, dentate gyrus (DG) and dorso-medial and ventro-lateral striatum (STR DM and STR VL) of R6/2 brains. A few aggregates were visualised in the IG as early as 14 days of age. In the hippocampus, inclusions were present at 19 days in CA1, whereas in the CA3 a few aggregates were visible at 29 days of age. In the dentate gyrus, MW8-positive aggregates were present at 26 days. In the striatum, Htt aggregates could be seen at 26 days of age in STR DM and STR VL. The number of aggregates in all the regions increased with time from the day of appearance. Scale bar = 50 µm.