N-myc Downstream Regulated Gene 1 (NDRG1) Promotes Metastasis of Human Scirrhous Gastric Cancer Cells through Epithelial Mesenchymal Transition
Figure 3
Comparison of biological characteristics between highly metastatic 58As1 and its NDRG1 knockdown cell lines.
(A) Cell proliferation in RPMI 1640 containing 10% FBS was followed after seeding 5×104 cells/dish on day 0. Doubling times were similar for As1/Mock3(black), As1/Sic50(gray) and As1/Sic54(white) cells (26–30 hr). (B) Cell morphology of As1/Mock3, As1/Sic50 and As1/Sic54. Typical cell morphology in culture shows As1/Mock3 suspension-type cell growth and attached layer-type cell growth of both NDRG1 knockdowned cell lines. (C) Representative images of colonies of As1/Mock3, As1/Sic50 and As1/Sic54 when incubated for 14 days in soft agar (left panel). Quantitative analysis of colony formation activity by three cell lines when 5 dishes for each line were scored (right panel). Significant differences (*p<0.01) in colony number between 58As1 and its NDRG1 knockdown cell lines.