Potential Role for PAD2 in Gene Regulation in Breast Cancer Cells
Figure 6
Knockdown of endogenous PAD2 in MCF-7 cells alters cellular proliferation rates versus control cells.
Equal numbers of shRNA control and PAD2 knockdown MCF-7 cells were plated in 96-well plates and allowed to grow for 24, 48, or 72 hours. At time of harvest, dye solution from MTT assay kit was allowed to incubate with cells for one hour at which point solubilization reagent was added and resulting formazan product measured at 570 nm. Data represent the means ± SEM of three independent experiments performed in triplicate. Values represent the means ± SEM.