Potential Role for PAD2 in Gene Regulation in Breast Cancer Cells
Figure 2
PAD2 is detected in the cytoplasm and nucleus of human breast luminal epithelial cells.
(A) PAD2 is expressed in luminal but not myoepithelial human breast cells. Normal human breast tissue sections were probed with anti-PAD2 (green fluorescent signal), anti-cytokeratin (luminal marker - red fluorescent signal), and anti-p63 (myoepithelial marker - red fluorescent signal) antibodies or equal concentration of rabbit and mouse IgG as a control and nuclei are stained with DAPI. (B) A portion of PAD2 staining in human breast epithelial cells is detected in the nucleus. Normal human breast tissue sections were probed with anti-PAD2 (green fluorescent signal) and nuclei are stained with DAPI. The white arrow illustrates a breast epithelial cell with robust PAD2 staining in the nucleus.