Localization and Expression of Zonula Occludins-1 in the Rabbit Corneal Epithelium following Exposure to Benzalkonium Chloride
Figure 7
Acute effect of BAC on the expression of ZO-1 mRNA and protein in the rabbit corneal epithelium.
(A) Corneal epithelial cells isolated from a control eye or from eyes treated with 0.005%, 0.01% or 0.02% BAC were subjected to western blot analysis with antibodies to ZO-1 or β actin (loading control). (B) Qutantitative analysis of ZO-1 band intensity in bolts similar to those shown in (A). Data were normalized by the corresponding β actin band intensity and mean ± SE of values from three eyes per group. (C) Corneal epithelial cells isolated from a control eye or from eyes treated with 0.005%, 0.01% or 0.02% BAC were subjected to RT-PCR analysis of ZO-1 mRNA. (D) Quantitative analysis of ZO-1 band intensity in gel similar to that shown in (A). Data were normalized by the corresponding G3PDH band intensity and are mean±SE of values from three eyes per group. Topical application of BAC had no significant effect on the amount of ZO-1 mRNA and protein (Dunnett test).