GLI2 Regulates TGF-β1 in Human CD4+ T Cells: Implications in Cancer and HIV Pathogenesis
Figure 4
GLI2 Knockdown in CD4+CD25+FoxP3+ Treg Decreases Levels of TGF-β1 mRNA.
CD4+CD25+FoxP3+ Treg were enriched from PBMC and then incubated for 72 hours with non-targeting (NT) or GLI2 siRNA. The cells were then stimulated (1 αCD3/αCD28 bead: 1 cell) for an additional 24 hours. RT-PCR was used to measure TGF-β1 mRNA levels. Relative TGF-β1 or GLI1 mRNA was normalized to 18S rRNA. (A) GLI2 knockdown in Treg significantly decreased TGF-β1 transcription (n = 7, * p = 0.0189). (B) GLI1 mRNA was also measured as a control GLI2-regulated gene; showing that knockdown of GLI2 in Treg also diminished GLI1 mRNA (n = 5, ** p = 0.0278). (C) The sequence of the GLI2 siRNA was scrambled (SCRAM) as an additional GLI2 siRNA negative control and was not significantly different from the non-targeting control.