Signal 3 Cytokines as Modulators of Primary Immune Responses during Infections: The Interplay of Type I IFN and IL-12 in CD8 T Cell Responses
Figure 2
Analysis of P14 CD8 T cells in the context of a rVSVGP infection.
P14.WT, P14.IL-12RKO, P14.IFNARKO or P14.DOKO T cells were transferred into B6 mice followed by infection with rVSVGP. Analysis of spleen cells at day 5 after infection is shown. A, Relative and total P14 T cell numbers per spleen is shown. B, Analysis of cell division by BrdU incorporation. After adoptive transfer, recipient mice were treated with 2 mg of BrdU i.p. on day 4. BrdU staining of P14.WT or P14.DOKO T cells gated on CD8+ T cells on day 5 in the spleen is shown. Numbers in brackets indicate percentages of BrdU+ cells of total P14 T cells. C, Analysis of KLRG1 and CD127 expression on P14 T cells. Plots are gated on CD8+, Thy1.1+ (P14) cells. D, Statistical analysis of T cell differentiation. Percentages of KLRG1 and CD127 positive P14 T cells of total P14 T cells are depicted. E, Cytolytic activity of P14 T cells was determined five days post infection by a 51Cr-release assay. Gp33-41 peptide-loaded or adeno peptide-loaded EL-4 cells (control) were used as targets. Equal numbers of purified P14 T cells were used as effectors. Expression of IFN-γ (F) and TNF-α (G) of P14 T cells. Percentages of cytokine positive P14 T cells of total P14 T cells, as well as MFI of cytokine expression are indicated. Values are expressed as mean ± SEM (n = 3). *p<0,01. Results are representative of three independent experiments.