The Transcription Factor c-Jun Protects against Liver Damage following Activated β-Catenin Signaling
Figure 5
Loss of c-Jun affects the expression of genes related to growth factor signaling and Akt.
(A) Hepatic expression of the indicated genes at 6 days upon Cre induction was determined by qPCR and is given as relative expression compared to control livers (n = 5 livers/genotype); *, P≤0.05. (B) Expression of the indicated proteins in total liver lysates of mice with the indicated genotypes was determined by immunoblotting. Ir, Insulin receptor. β-Actin was used as control. Samples from livers with strongly increased hepatocyte vacuolization are indicated. (C) Hepatic glycogen content and serum insulin concentrations were analyzed 6 days after Cre induction (n = 4–11/genotype); *, P≤0.05. (D) BiP expression predominantly occurred in vacuolated hepatocytes in DKO mice as determined by immunohistochemistry.