CD154 Blockade Alters Innate Immune Cell Recruitment and Programs Alloreactive CD8+ T Cells into KLRG-1high Short-Lived Effector T Cells
Figure 5
Anti-CD154 and DST distinctly alter alloreactive CD8+ T cell programming into cytokine-producing effector cells.
B6-Ly5.2/Cr mice were transplanted with BALB/c skin grafts and were treated with 107 BALB/c DST and/ or anti-CD154 mAb, where indicated. A. Representative flow plots of TNF and IFN-γ producing CD8+ T cells after ex vivo restimulation with BALB/c splenocytes, isolated from spleens of mice at day 7 post-transplantation. B. Absolute count of total IFN-γ producing CD8+ T cells in the spleen over time following ex vivo restimulation. C. Pie charts represent total activated CD44high CD62Llow CD8+ T cells. The black wedges represent the frequency of activated CD44high CD62Llow CD8+ T cells that produce IFN-γ on day 7 post-transplantation (p<0.05). D. Pie charts represent all IFN-γ producing CD8+ T cells. The striped wedges represent the IFN-γ-only producing population and black segments represent the TNF/IFN-γ double producing population in untreated vs. DST treated mice on day 7 (p = 0.0028). Data are summary of two experiments with three mice per group. Values are mean ± SEM. *p<0.05, **p<0.01, ***p<0.001.