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An Automated Flow for Directed Evolution Based on Detection of Promiscuous Scaffolds Using Spatial and Electrostatic Properties of Catalytic Residues

Figure 2

Superimposition of pseudechetoxin (PDB id: 1CFE, in green) and P14a (PDB id: 1B0F, in blue) and using TopMatch.

The overlapping regions are colored red and orange for pseudechetoxin and P14a respectively. It is known that pseudechetoxin has elastase activity.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0040408.g002