The Two-Component Signal Transduction System ArlRS Regulates Staphylococcus epidermidis Biofilm Formation in an ica-Dependent Manner
Figure 1
Effect of ArlRS deficiency on S.epidermidis growth and biofilm formation in vitro.
(A)Semi-quantitative detection of biofilms formed by SE1457, WW06, ParlS and ParlRS over a 24 h period in 96-well polystyrene microtiter plates: The biofilms were stained with 2% crystal violet. (B) The crystal violet was dissolved in ethanol and the absorbance was determined at 570 nm. The mean value ± standard deviation for each strain is shown. (C) Growth curves of S. epidermidis strains: Overnight cultures of SE1457, WW06, ParlS and ParlRS were diluted to OD595 = 0.08 in 150 ml fresh TSB and incubated at 37°C with shaking.The value of OD595 was monitored hourly. The curves represent the means of three independent experiments with standard deviation (SD). Staphylococcus epidermidis strain ATCC12228, a non-biofilm forming control; RP62A, a biofilm positive control.