MMP-8 Deficiency Increases TLR/RAGE Ligands S100A8 and S100A9 and Exacerbates Lung Inflammation during Endotoxemia
Figure 3
Differential expression of S100 proteins between genotypes.
N = 7/group. The differences in S100A6 levels were not confirmed (A, p = 0.45 in the ANOVA). However, S100A8 (B) and S100A9 (C) protein levels increased after LPS injection in both genotypes (p<0.05 in all post-hoc tests). Mice lacking MMP-8 showed significantly higher levels of these two proteins than their wildtype counterparts (p<0.01 and p<0.001 for the differences between genotypes in S100A8 and S100A9 respectively). Panel D shows representative western blots. However, S100A8 and S100A9 gene expression was not different between genotypes during endotoxemia (n = 6/group, E). To discard systemic differences in alarmins, levels of S100A8 and S100A9 were measured in spleen homogenates (n = 7/group) and serum (n = 4/group) from LPS-treated animals, with no significant differences between genotypes (F). *p<0.05 in post-hoc test.