Doxycycline Stabilizes Vulnerable Plaque via Inhibiting Matrix Metalloproteinases and Attenuating Inflammation in Rabbits
Figure 6
Immunofluorescence and western blot analysis of MMP-2 and MMP-9 expression in vitro.
Panel A shows the immunofluorescence images of MMP-2 in macrophages receiving doxycycline, simvastatin or no treatment. Panel B shows the immunofluorescence images of MMP-9 in macrophages receiving doxycycline, simvastatin or no treatment. Panel C shows western blot analysis of MMP-2 and MMP-9 expression. Panel D and E are the quantitative analysis of Panel C. Blue color represents DAPI staining, green color MOMA-2 staining and red color MMP-2 or MMP-9 staining. Bars = 20 µm. Group D: doxycycline-treated group; Group S: simvastatin-treated group; Control: control group. **P<0.01, vs. Control group.