High-Throughput Multilocus Sequence Typing: Bringing Molecular Typing to the Next Level
Figure 2
The workflow for profiling 96 bacterial isolates with HiMLST.
MLST target genes are amplified by a two-step PCR in multi-well plates. These amplicons are pooled, quantified, clonally amplified by emulsion PCR and sequenced by NGS. The workflow ends with the ST profiling of the individual bacterial isolate. Figures in squares indicate the hands-on time for each individual step.