Activated Human CD4+CD45RO+ Memory T-Cells Indirectly Inhibit NLRP3 Inflammasome Activation through Downregulation of P2X7R Signalling
Figure 4
IL10 does not mediate the observed suppression of active IL-1β release.
a) memory CD4+CD45RO+ T-cells cultured overnight in the presence of monocytes with or without αCD3 and/or IFNβ were restimulated with PMA/Ionomycin in the presence of Brefeldin A for 4 h and stained for IL-10 and IFNγ (representative of 3 independent experiments); b) upregulation of IL10 mRNA expression in resorted memory T-cells was confirmed by qPCR (n = 3; n.d. = not detected); c) inhibition of IL-10 (10 ug/ml) and/or IFNγ (10 ug/ml) by specific blocking antibodies did not affect the suppressive effect on IL-1β release by activated T-cells in the presence of IFNβ (n = 4) (% suppression is calculated by subtracting from 1 the ratio of IL-1β release from activated T-cells in the presence of IFNβ and non-activated T-cells in the absence of IFNβ and multiplying by 100; **p<0.01 employing repeated measures ANOVA with post-hoc Bonferroni adjustment for multiple comparisons to avoid random correlations).