Cooperative Interaction between the MUC1-C Oncoprotein and the Rab31 GTPase in Estrogen Receptor-Positive Breast Cancer Cells
Figure 3
E2-induced Rab31 expression is dependent on MUC1.
A. RNA from MCF-7 (left) and ZR-75-1 (right) cells stimulated with E2 for the indicated times was analyzed by qRT-PCR. The results (mean±SD of three determinations) are expressed as relative Rab31 mRNA levels compared to that obtained in unstimulated cells. B and C. RNA from the indicated MCF-7 (B) and ZR-75-1 (C) cells stimulated with E2 for 0, 6 or 24 h was analyzed by qRT-PCR. The results (mean±SD of three determinations) are expressed as relative Rab31 mRNA levels compared to that obtained in the unstimulated vector cells. D. Lysates from MCF-7 (left) and ZR-75-1 (right) cells stimulated with E2 for 0, 6 and 24 h were immunoblotted with the indicated antibodies. E. Lysates from the indicated MCF-7 cells stimulated with E2 for 0, 6 and 24 h were immunoblotted with the indicated antibodies.