Antitumor Activities of Human Placenta-Derived Mesenchymal Stem Cells Expressing Endostatin on Ovarian Cancer
Figure 3
Migratory ability of transduced hpMSCs in vitro and in vivo.
A. Migration of hpMSCs/hpMSCs-Ad-Endo cells through an 8-um Millicell membrane. HpMSCs were cultured in 24-well plate onto 8-um pore-sized membranes, and A2780s or 293 cells were cultured in the lower chamber. The cells migrated to the lower side of the membrane were labeled with methyl violet. Original magnification×400. B. Induction of hpMSCs/hpMSCs-Ad-Endo migration stimulated by A2780s or 293 cells. Compared with 293 cells, A2780s cells promoted migration of hpMSCs//hpMSCs-Ad-Endo significantly. Data shown as means ± SD. C. To verify the specific homing property of transducted mesenchymal to tumor site, we transducted hpMSCs with adenovirus carrying GFP and injected i.p. to nude mice with established peritoneal ovarian tumor. After 3 days and 2 weeks of i.p. injection, mice were sacrificed and tumor nodules and organs were collected. Left panel: 3 days after i.p. injection, GFP signal (arrow) were detected at tumor periphery. Right panel: 2 weeks after i.p. injection, positive GFP signal were detected in the tumor parenchymal (arrow) which indicated hpMSCs persist in the xenograft and can integrate into tumor focus. There is no positive GFP signal detected in the organs.