Xenopus as a Model System for the Study of GOLPH2/GP73 Function: Xenopus golph2 Is Required for Pronephros Development
Figure 6
Xenopus golph2 is required for pronephros development. A.
Schematic diagram of the pronephros with a summary of the pronephric marker genes that were used (lateral view, anterior towards the left). At stage 38, the pronephros is subdivided into distinct segments: the glomus, proximal tubule (PT1, PT2, PT3), intermediate tubule (IT1, IT2), distal tubule (DT1, DT2) and pronephric duct (PD). B–M’. Expression of the marker genes; the orientations of panels B to M show a lateral view, the anterior towards the right; the orientations of panels B’ to M’ show a lateral view, the anterior towards the left. A total of 32 ng of Control or golph2 morpholino was injected into a single ventral blastomere of 4-cell-stage embryos from the lateral region; whole-mount in situ hybridization analysis was performed on stage 38. (B–C’) Nephrin; (a–c) transverse section of the embryos in panels B’ and C’; (D–E’) atp1b1; (F–G’) SGLT-1K; (H–I’) ClC-K; (J–K’) NKCC2; (L–M’) NBC1.